Volume 8, Issue 1, February 2020, Page: 12-16
Highly Anticancer and Moderate Thrombolytic Property of Accacia rugata of Mimosaceae Family
Bibi Humayra Khanam, Department of Pharmacy, University of Chittagong, Chittagong, Bangladesh
Ahmed Rakib, Department of Pharmacy, University of Chittagong, Chittagong, Bangladesh
Farhana Binta Faiz, Department of Pharmacy, University of Chittagong, Chittagong, Bangladesh
Md. Giash Uddin, Department of Pharmacy, University of Chittagong, Chittagong, Bangladesh
Mohammed Kamrul Hossain, Department of Pharmacy, University of Chittagong, Chittagong, Bangladesh
Ramiz Ahmed Sultan, Department of Pharmacy, University of Chittagong, Chittagong, Bangladesh
Received: Feb. 2, 2020;       Accepted: Feb. 26, 2020;       Published: Apr. 7, 2020
DOI: 10.11648/j.jps.20200801.12      View  400      Downloads  99
Medicinal plants containing potent bioactive compound effective in treating many diseases exert different pharmacological action. This study designed to evaluate the cytotoxic and thrombolytic activity of Acacia rugata of Mimosaceae family. The cytotoxic and thrombolytic activity was evaluated by brine-shrimp lethality bioassay and in-vitro clot lysis method. Methanol, petroleum-ether, n-hexane, chloroform and dichloromethane fraction leaves (MEL, PETFL, n-HxFL, CHFL, DCMFL), methanol and n-hexane fraction of fruits (MEF, n-HxFF) and methanol fraction of bark (MEB) were used to evaluate cytotoxicity of the plant. Each extracts showed significant cytotoxic property. The LC50 values of MEL, PETFL, n-HxFL, CHFL, DCMFL, MEF, n-HxFF, MEB were observed 1.436, 0.039, 0.974, 0.626, 0.121, 0.176, 0.865, 0.081 µg/ml when compared to standard vincristine (positive control) which LC50 value was 0.049 µg/ml. In thrombolytic activity evaluation 20 mg/ml, 10 mg/ml, 5 mg/ml and 2.5 mg/ml dose of MEL showed 40.52 ± 2.91, 35.09 ± 2.71, 31.96 ± 2.02 and 24.91 ± 3.05% clot lysis respectively while 0.9% NaCl solution (negative control) and standard streptokinase (positive control) exhibited 7.41 ± 1.73% and 48.91 ± 3.52% of clot lysis. It can be assumed that different solvent extracts of A. rugata have important cytotoxic and thrombolytic activity as compared to standard compounds.
Cytotoxic, Thrombolytic, Streptokinase, Vincristine, % of Clot Lysis, Acacia rugata
To cite this article
Bibi Humayra Khanam, Ahmed Rakib, Farhana Binta Faiz, Md. Giash Uddin, Mohammed Kamrul Hossain, Ramiz Ahmed Sultan, Highly Anticancer and Moderate Thrombolytic Property of Accacia rugata of Mimosaceae Family, Journal of Plant Sciences. Vol. 8, No. 1, 2020, pp. 12-16. doi: 10.11648/j.jps.20200801.12
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Sen N, Bulbul L, Hussain F, Amin MT. (2016). Assessment of thrombolytic, membrane stabilizing potential and total phenolic content of Typha elephantina Roxb. Journal of Medicinal Plants Research, 10: 669-675.
Prema R, Sekar DS, Sekhar KB, Jeevanandham S. (2012). In vitro cytotoxicity study on combined plants extracts (Cissus quadrangularis and Aegle marmelos). European Journal of Experimental Biology, 2: 882-8.
Wittstock U, Gershenzon J. (2002). Constitutive plant toxins and their role in defense against herbivores and pathogens. Current Opinion in Plant Biology, 5 (4): 300-307.
Calixto JB. (2000). Efficacy, safety, quality control, marketing and regulatory guidelines for herbal medicines (phytotherapeutic agents). Brazilian Journal of Medical and Biological Research, 33 (2): 179-189.
Altemimi A, Lakhssassi N, Baharlouei A, Watson DG, Lightfoot DA. (2017). Phytochemicals: Extraction, isolation, and identification of bioactive compounds from plant extracts. Plants, 6 (4): 42.
Rai PK, Jaiswal D, Singh RK, Gupta RK, Watal G. (2008). Glycemic properties of Trichosanthes dioica leaves. Pharmaceutical Biology, 46 (12): 894-899.
Islam SM, Ahmed KT, Manik MK, Wahid MA, Kamal CS (2013). A comparative study of the antioxidant, antimicrobial, cytotoxic and thrombolytic potential of the fruits and leaves of Spondias dulcis. Asian Pacific journal of Tropical Biomedicine, 3 (9): 682-91.
Greenwell M, Rahman PK. (2015). Medicinal plants: their use in anticancer treatment. International Journal of Pharmaceutical Sciences and Research, 6 (10): 4103.
Newman DJ. (2008). Natural products as leads to potential drugs: an old process or the new hope for drug discovery?. Journal of Medicinal Chemistry, 51 (9): 2589-2599.
Newman DJ, Cragg GM, Snader KM. Natural products as sources of new drugs over the period 1981− 2002. (2003). Journal of Natural Products, 66 (7): 1022-37.
Apu AS, Muhit MA, Tareq SM, Pathan AH, Jamaluddin AT, Ahmed M. (2010). Antimicrobial Activity and Brine Shrimp Lethality Bioassay of the Leaves Extract of Dillenia indica Linn. Journal of Young Pharmacists, 2 (1): 50-3.
Ali MS, Amin MR, Kamal CM, Hossain MA. (2013). In vitro antioxidant, cytotoxic, thrombolytic activities and phytochemical evaluation of methanol extract of the A. philippense L. leaves. Asian Pacific Journal of Tropical Biomedicine, 3 (6): 464-9.
Kamal AM, Chowdhury KA, Rana MM, Islam A, Khan EA, Haque MA, Chy MM. (2015). Study of cytotoxic, thrombolytic and anthelmintic activity of extract of Neolamarckia cadamba (Roxb.) leave. European Journal of Medicinal Plants, 1-9.
Hunt BJ. (2008). Awareness and politics of venous thromboembolism in the United Kingdom. Arteriosclerosis, Thrombosis, and Vascular Biology, 28 (3): 398-399.
Aasim M, Khawar KM, Ahmed SI, Karataş M. (2019). Multiple uses of some important aquatic and semiaquatic medicinal plants. In Plant and Human Health, 2: 541-577.
Dhiman N, Patial V, Bhattacharya A. (2018). The current status and future applications of hairy root cultures. In Biotechnological Approaches for Medicinal and Aromatic Plants, 87-155.
Rahaman MS, Rahaman MS, Bari MA, Barua R, Islam JM, Islam MS, Khan MA. (2019). An approach to evaluate anti-arthritic and thrombolytic activity of different parts of Solanum torvum Sw. (Solanaceae) and Smilax zeylanica L. (Liliaceae). Journal of Drug Delivery and Therapeutics, 9 (4-s): 155-64.
Zohora FT, Islam SN, Khan SA, Hasan CM, Ahsan M. (2019). Antioxidant, cytotoxic, thrombolytic and antimicrobial activity of zanthoxylum rhetsa root bark with two isolated quinolone alkaloids. Pharmacology & Pharmacy, 10 (3): 137-45.
Ghani A. 2003. Medicinal plants of Bangladesh with chemical constituents and uses. 2nd edition; published by Asiatic Society of Bangladesh, 331-332.
Ezeja MI, Omeh YS, Ezeigbo II, Ekechukwu A. (2011). Evaluation of the analgesic activity of the methanolic stem bark extract of Dialium guineense (Wild). Annals of Medical and Health Sciences Research, 1 (1): 55-62.
Ahmed S, Rakib A, Islam MA, Khanam BH, Faiz FB, Paul A, Chy MN, Bhuiya NM, Uddin MM, Ullah SA, Rahman MA. (2019). In vivo and in vitro pharmacological activities of Tacca integrifolia rhizome and investigation of possible lead compounds against breast cancer through in silico approaches. Clinical Phytoscience, 5 (1): 36.
Islam MA, Sayeed MA, Khan G, Mosaddik MA, Bhuyan MS. (2002). Terpenes from bark of Zanthoxylum budrunga and their cytotoxic activities. Revista Latinoamericana De Quimica, 30: 24-28.
Islam MR, Naima J, Proma NM, Hussain MS, Uddin SN, Hossain MK. (2019). In-vivo and in-vitro evaluation of pharmacological activities of Ardisia solanacea leaf extract. Clinical Phytoscience, 5 (1): 32.
Déciga-Campos M, Rivero-Cruz I, Arriaga-Alba M, Castañeda-Corral G, Angeles-López GE, Navarrete A, Mata R. (2007). Acute toxicity and mutagenic activity of Mexican plants used in traditional medicine. Journal of Ethnopharmacology, 110 (2): 334-42.
Rakib A, Ahmed S, Islam MA, Haye A, Uddin SN, Uddin MM, Hossain MK, Paul A, Emran TB. (2020). Antipyretic and hepatoprotective potential of Tinospora crispa and investigation of possible lead compounds through in silico approaches. Food Science & Nutrition, 8 (1): 547-56.
Kamal AM, Chowdhury KA, Shill LK, Hossain MR, Islam N, Anaytulla IA, Hassan MF. (2015). Phytochemical screening, cytotoxic and thrombolytic activity of extract of Brassica oleracea flower (cauliflower). Global Journal of Pharmacology, 9 (1): 115-20.
Zaman R, Parvez M, Jakaria M, Sayeed MA, Islam M. (2015). In vitro clot lysis activity of different extracts of mangifera sylvatica roxb. Leaves. Research Journal of Medicinal Plant, 9 (3): 135-140.
Oukerrou MA, Tilaoui M, Mouse HA, Leouifoudi I, Jaafari A, Zyad A. (2017). Chemical composition and cytotoxic and antibacterial activities of the essential oil of Aloysia citriodora palau grown in Morocco. Advances in Pharmacological Sciences, 2017.
Monga J, Chauhan CS, Sharma M. (2011). Human epithelial carcinoma cytotoxicity and inhibition of DMBA/TPA induced squamous cell carcinoma in Balb/c mice by Acacia catechu heartwood. Journal of Pharmacy and Pharmacology, 63 (11): 1470-1482.
Prasad S, Kashyap R S, Deopujari JY, Purohit HJ, Taori GM, Daginawala HF. (2007). Effect of Fagonia arabica (Dhamasa) on in vitro thrombolysis. BMC Complementary and Alternative Medicine, 7 (1): 36.
Hussain MS, Hossain MS, Amin MT, Millat MS. (2016). In vitro thrombolytic potentials of methanolic extract of Vigna unguiculata Linn (seed). Journal of Pharmacognosy and Phytochemistry, 5 (3): 129.
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