Volume 7, Issue 6, December 2019, Page: 131-136
Effect of Different Carbon Sources on in vitro Shoot Regeneration and Multiplication of Cissampelos pareira (L.) - An Important Medicinal Plant
Cingithalam Munaswamy Reddy Narendra Reddy, Department of Biotechnology, School of Herbal Studies and Naturo Sciences, Dravidian University, Kuppam, A. P. India
Palyam Venkatesh Chaithanya Lakshmi, Department of Biotechnology, School of Herbal Studies and Naturo Sciences, Dravidian University, Kuppam, A. P. India
Varagiri Narayanachetty Swetha Prasuna, Department of Biotechnology, School of Herbal Studies and Naturo Sciences, Dravidian University, Kuppam, A. P. India
Mustrahally Raja Gopal, Department of Biotechnology, School of Herbal Studies and Naturo Sciences, Dravidian University, Kuppam, A. P. India
Gunda Sudhasri Purna, Department of Biotechnology, School of Herbal Studies and Naturo Sciences, Dravidian University, Kuppam, A. P. India
Bathula Srinivas, Department of Biotechnology, School of Herbal Studies and Naturo Sciences, Dravidian University, Kuppam, A. P. India
Received: Sep. 24, 2019;       Accepted: Oct. 17, 2019;       Published: Oct. 28, 2019
DOI: 10.11648/j.jps.20190706.11      View  26      Downloads  18
Abstract
An efficient in vitro protocol for shoot regeneration and production of multiple shoots of Cissampelos pareira (L) was developed. In the present study, the effect of different carbon sources, sucrose, fructose, maltose and glucose was investigated on in vitro shoot regeneration by using axillary bud of Cissampelos pareira (L). The frequency, growth and multiplication rate were highly influenced by the type and concentration of carbon sources used. The highest number of shoots (8.06±0.11) was observed on MS medium supplemented with 2% fructose and along with BAP 1.0mg/l and NAA 0.5mg/l and regeneration frequency 95%. The least number of shoots were obtained on MS medium supplemented with 4% maltose and (3.4±0.10) shoots and glucose (3.26±0.11). Among the different carbon sources used in the present study, fructose 2% proved to be a better choice for multiple shoot regeneration followed by Sucrose, maltose, and glucose from axillary bud explants of Cissampelos pareira. In vitro shoots were then excised from the shoot clumps and transferred to the rooting medium containing NAA, IBA (0.5-3.0mg/l). The well-rooted plantlets were then separated from the culture tubes and transferred into sterile soil and vermiculate (1:1) in the greenhouse. Finally, the hardened plants were transferred to the field environment for utmost survivability.
Keywords
Axillary Bud Explants, Carbon Sources, BAP-6-Benzyl Amino Purine, NAA-1-Napthalene Acetic Acid, Micro Propagation, Cissampelos pareira
To cite this article
Cingithalam Munaswamy Reddy Narendra Reddy, Palyam Venkatesh Chaithanya Lakshmi, Varagiri Narayanachetty Swetha Prasuna, Mustrahally Raja Gopal, Gunda Sudhasri Purna, Bathula Srinivas, Effect of Different Carbon Sources on in vitro Shoot Regeneration and Multiplication of Cissampelos pareira (L.) - An Important Medicinal Plant, Journal of Plant Sciences. Vol. 7, No. 6, 2019, pp. 131-136. doi: 10.11648/j.jps.20190706.11
Copyright
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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