Volume 3, Issue 5, October 2015, Page: 264-271
Purification and Toxicity Study of a Saponin from Seeds of Albizia odorata, a Fabaceae from Madagascar
Clara Fredeline Rajemiarimoelisoa, Department of Pharmacy, Faculty of Medicine, Antananarivo, University of Antananarivo, Madagascar
Danielle Aurore Doll Rakoto, Laboratory of Applied Biochemistry to Medical Sciences, Fundamental and Applied Biochemistry Department, Faculty of Sciences, University of Antananarivo, Antananarivo, Madagascar
Hanitra Ranjana Randrianarivo, Laboratory of Applied Biochemistry to Medical Sciences, Fundamental and Applied Biochemistry Department, Faculty of Sciences, University of Antananarivo, Antananarivo, Madagascar
Victor Louis Jeannoda, Laboratory of Applied Biochemistry to Medical Sciences, Fundamental and Applied Biochemistry Department, Faculty of Sciences, University of Antananarivo, Antananarivo, Madagascar
Received: Sep. 19, 2015;       Accepted: Oct. 7, 2015;       Published: Oct. 19, 2015
DOI: 10.11648/j.jps.20150305.14      View  4890      Downloads  150
In order to continue the research of natural compounds of interest such as pesticides and therapeutic molecules in endemic species of Albizia from Madagascar, potentials of Albizia odorata seed extract were assessed. A toxic saponin (saponoside), named Albodorine, was isolated by extraction with hot ethanol or distilled water followed by purification procedure comprising n-butanol partition, precipitation by aceton-diethyl ether (50/50), Sephadex LH-20 gel chromatography and silica gel chromatography. All these methods were guided by toxicity tests on mice and homogeneity tests by Thin Layer Chromatography (TLC). Albodorine was thermostable, soluble in water and organic solvents and tasted bitter. Its acidic hydrolysis released glucose, arabinose and rhamnose. Tested on different experimental animal models, it was toxic to warm and cold blooded animals. In mouse, when intraperitoneally administered, it caused acute intoxication mainly presented as hyperpnea, ataxia and terminal seizures before the animal died. Its LD50 was about 9 mg/kg of mouse body weight by intraperitoneal route. In different organs, it caused histopathological lesions characterized by vascular congestions and important hemorrhage in liver, lungs and kidneys. In vitro, it reduced the heart rate and force of contraction of isolated rat atria. It had hemolytic activity. Albodorine showed toxicological properties that could be exploited under certain conditions for the control of harmful organisms.
Albizia odorata, Toxin, Albodorine, Saponin, Hemolysis, Histopathology, Isolated Atria, Inotropic Effect
To cite this article
Clara Fredeline Rajemiarimoelisoa, Danielle Aurore Doll Rakoto, Hanitra Ranjana Randrianarivo, Victor Louis Jeannoda, Purification and Toxicity Study of a Saponin from Seeds of Albizia odorata, a Fabaceae from Madagascar, Journal of Plant Sciences. Vol. 3, No. 5, 2015, pp. 264-271. doi: 10.11648/j.jps.20150305.14
D. A. D. Rakoto, C. Rajemiarimoelisoa, R. Randrianarivo, D. Ramamonjison, C. Raheriniaina, N. Raharisoa and V. Jeannoda. Antimicrobial Activity of Some Endemic Species of Albizia (Fabaceae) from Madagascar, Asian Biotechnology and Development Review, 13 (2011) 53-60.
D. A. D. Rakoto, R. Randrianarivo, M. El-Yachouroutui, A. A. Arisoa, N. Raharisoa, N. Rakotondrasoa, P. Raoniharisoa and V. Jeannoda. Effects of Extracts from Albizia (Fabaceae) Endemic Species of Madagascar on Vegetable Seedling Development, J. Chem. Chem. Eng. 6 (2012) 313-322.
L. Randriamampianina, A. Offroy, L. Mambu, H. R. Randrianarivo, D. Rakoto, V. Jeannoda, C. Djediat, S. Puiseux Dao and M. Edery, Marked Toxicity of Albizia bernieri Extracts on Embryo-larval Development in the Medaka Fish (Oryzias latipes), Toxicon 64 (2013) 29-35.
H. R. Randrianarivo, A. R. Razafindrakoto, H. C. Ratsimanohatra, L. J. Randriamampianina, C. F. Rajemiarimoelisoa,, L. Ramamonjisoa, D. Ramanitrahasimbola, D. A. D. Rakoto, and V. L. Jeannoda. Toxic effects of seed methanolic extracts of endemic Albizia species (Fabaceae) from Madagascar on animals, Journal of life Sciences, 8 (8) (2014) 679-689.
H. R. Randrianarivo, H. C. Ratsimanohatra, A. R. Razafindrakoto, C. F. Rajemiarimoelisoa, L. J. Randriamampianina, L. Ramamonjisoa, D. A. D. Rakoto and V. L. Jeannoda. Phytotoxic property of seed methanolic extracts from Albizia (Fabaceae) endemic species of Madagascar. Journal of Plant Sciences 2 (6) (2014), 256-265.
C. F. Rajemiarimoelisoa, C. B. L. Pellissierb, M. Peuchmaurb, H. R. Randrianarivo, D. A. D. Rakoto, V. L. Jeannoda and A. Boumendjel. Isolation of β-carbolines from pods of Albizia polyphylla. Natural Product Research (in press).
D. J. Du Puy, J. N. Labat, R. Rabevohitra, J.-F. Villiers, J. Bosser, J. Moat. The Leguminosae of Madagascar. Royal Botanic Gardens, Kew, Richmond, United Kingdom, (2002) pp. 243–288.
A. P. Barbosa. Pharmacologically active saponins from the genus Albizia (Fabaceae). International Journal of Pharmacy and Pharmaceutical Sciences, 6 (11) (2014) 32-36.
J. T. Grade, B. L. Arble, R. B. Weladji and P. V. Damme. Anthelmintic efficacy and dose determination of Albizia anthelmintica against gastrointestinal nematodes in naturally infected Ugandan .sheep. Vet Parasitol 157 (2008) 267-74.
I. M Nurul, H. Mizuguchi, M. Shahriar, P. Venkatesh, K. Maeyama, P. K. Mukherjee, Hattori M, M. S. Choudhuri, N. Takeda and H Fukui. Albizia lebbeck suppresses histamine signaling by the inhibition of histamine H1 receptor and histidine decarboxylase gene transcriptions. Int Immunopharmacol 11 (2011) 1766-72.
L. Zheng, J. Zheng, Y. Zhao, B. Wang, L. Wu and H. Liang, Three anti-tumor saponins from Albizia julibrissin. Bioorg. Med. Chem. Lett. 16 (2006) 2765-2768.
K. Zou, Y.-Y. Zhao and R.-Y. Zhang. A cytotoxic saponin from Albizia julibrissin. Chem. Pharm. Bull. 54 (2006) 1211-1212.
S. Krief, O. Thoison, T. Sévenet, R. Wrangham and C. Lavaud. Triterpenoid saponin anhtranilates from Albizia grandibracteata leaves ingested by primates in Uganda, J. Nat. Prod. 68 (2005) 897-903.
S. G. Cao, A. Norris, J. S. Miller, F. Ratovoson, J. Razafintsalama, R. Andriantsiferana, V. E. Rasamison, K. Tendyke, T. Suh, D. G. I. Kingston, Cytotoxic triterpenoid saponins of Albizia gummifera from Madagascar rain forest, J. Nat. Prod. 70 (2007) 361-366.
G. M. Rukunga and P. G. Waterman, Triterpenes of Albizia versicolor and Albizia schimperana stem barks. Fitoterapia 72 (2001) 188-190.
P. Avato, R. Bucci, A. Tava, C. Vitali, A. Rosato, Z. Bialy and M. Jurzysta Antimicrobial activity of saponins from Medicago sp.: Structure-activity relationship. Phytotherapy Research 20 (2006) 454-457.
A. Tava and P. Avato. Chemical and biological activity of triterpene saponins from Medicago species. Natural Product Communications 1 (2006) 1159-1180.
O. P. Noté, D. Jihu, C. Antheaume, M. Zeniou, D. E. Pegnyemb, D. Guillaume, H. Chneiwess, M. C. Kilhoffer and A. Lobstein. Triterpenoid saponins from Albizia boromoensis Aubrev. & Pellegr. Phytochemistry Letters 11 (2015) 37–42.
W. Cai, Y. Li, Q. G Yi, F. Xie, B. Du, L. Feng and L. Qiu. Total saponins from Albizia julibrissin inhibit vascular endothelial growth factor-mediated angiogenesis in vitro and in vivo. Molecular Medicine Reports 11 (2015) 3405-3413.
R. G. Powell. Plant seeds as sources of potential industrial chemicals, pharmaceuticals, and pest control agents. J. Nat. Prod. 72 (2009) 516–523.
V. Ranjamala. Etude du genre Albizia comme essences à usages multiples dans la région d’Ankarafantsika. Mémoire de DEA de Biologie végétale, Faculté des Sciences, Université d’Antananarivo (1995) p91.
E. H. S. Fong, M. Tin-Wa, N. R. Farnsworth and R. H. Dobberstein. Phytochemical screening methods. Rev. Department of pharmacognosy and pharmacology, college of pharmacy, University of Illinois (1977).
G. B. Marini-Bettolo, S. Nicoletti and M. Patami, Plant screening by chemical and chromatographic procedure under field conditions, J. Chromatogr. 218 (1981) 113-217.
Z. Dische and L. B. Shettles. A specific color reaction of methylpentoses and a spectrophotometric micromethod for their determination. J. Biol. Chem. 175 (1948) 595-603.
P. Louisot. Biochimie générale et médicale : introduction chimique à la biochimie, glucides, acides nucléiques, vitamines, coenzymes, lipides et dérivés isopréniques, amino-acides, peptides, protéines. Simep, (1989) p488.
J. Bruneton. Pharmacognosie, phytochimie, plantes médicinales (4e éd.). Techniques et Documentation (Lavoisier), Paris (2009) 1268 p.
E. De Geyter, E. Lambert, D. Geelen and G. Smagghe. Novel Advances with Plant Saponins as Natural Insecticides to Control Pest Insects. Pest Technology 1(2) (2007) 96-105.
A. E. Osbourn. Saponins in cereals. Phytochemistry 62 (2003) 1-4.
A. I. Ahmed, W. Oleszek, A. Stochmal, C. Pizza and S. Piacente. Steroidal saponins from the aerial part of Trubulus pentandrus Forssk. Phytochemistry 65(21) (2004) 2935-2945.
F. Q. Xu, H. M. Zhong, H. Y. Liu, H. Q. Liu and C. X. Chen. Steroidal saponins from Lysimachia Paridiformis. J Asian Nat Prod Res 9 (2007) 493–497.
A. Zamilpa, J. Tortoriello, V. Navarro, G. Delgado and L. Alvarez. Five new steroidal saponins from Solanum chrysotrichum leaves and their antimycotic activity. J. Nat. Prod., 65 (2002) 1815.
G. Francis, Z. Kerem, H. P. S. Makkar and K. Becker. The biological action of saponins in animal systems: a review. British Journal of Nutrition 88 (2002) 587-605.
A. Kohara, C. Nakajima, S. Yoshida and T. Muranaka. Characterization and engineering of glycosyl transferases responsible for steroid saponin biosynthesis in Solanaceous plants. Phytochemistry 68 (2007) 478-486.
P. K Lunga, X.-J. Qin, X. W Yang, J.-R. Kuiate, Z. Z. Du and D. G. Antimicrobial steroidal saponin and oleanane-type triterpenoid saponins from Paullinia pinnata. BMC Complementary and Alternative Medicine 14 (2014) 369.
J. P. Vincken, L. Heng, A. de Groot and H. Gruppen. Saponins, classification and occurrence in the plant kingdom. Phytochemistry 68 (2007) 275-297.
S. L. Deore, S. S. Khadabadi, K. P. Chittam, P. G. Bhujade, T. P. Wane, Y. R. Nagpurkar, P. D. Chanekar and R. G. Jain. Properties A. D. pharmacological applications of saponins. Pharmacologyonline 2 (2009) 61-84.
H. D. Neuwinger. African ethnobotany. Poisons and drugs: chemistry, pharmacology, toxicology. Germany: Chapman and Hall, (1996) p 939.
Browse journals by subject