Volume 3, Issue 5, October 2015, Page: 259-263
Evaluation of the Antibacterial Activity of Seed Coat Extracts of Roselle (Hibiscus Sabdariffa L.) on Selected Antibiotic Resistant Bacterial Species
Orowale Kehinde Nathaniel, Department of Plant Science and Technology, Faculty of Natural Science, University of Jos, Plateau State, Nigeria
Agina S. E., Department of Plant Science and Technology, Faculty of Natural Science, University of Jos, Plateau State, Nigeria
Received: Sep. 18, 2015;       Accepted: Sep. 26, 2015;       Published: Oct. 15, 2015
DOI: 10.11648/j.jps.20150305.13      View  3220      Downloads  64
This research study is on the evaluation of the antibacterial activity of Roselle (Hibiscus sabdariffa L.) seed coats using Aqueous, Ethanol, Hexane and Methanol as solvent. it was examined against selected antibiotic resistant bacterial species (Escherichia coli, Staphyloccocus aureus, Streptococcus pneumonia, Klebsiella aerogenes, Salmonella spp, Pseudomonas aeruginosa). The result evaluates the zones of inhibitions shown by each of the extracts against the test organisms which later revealed that at the various concentrations used, none of the seed coat extracts showed any inhibitory properties against the test microorganism. The phytochemical analysis of the seed coat of H. sabdariffa revealed the presence of bioactive compounds such as Alkaloids, carbohydrates, steroids, Cardiac glycosides. The result from the study reveals that the seed coat extracts of Hibiscus sabdariffa does not possess any inhibitory activity against the bacterial species examined. Notwithstanding, other areas should be exploited for its possible application.
Hibiscus sabdariffa, Seed Coat Extracts, Antibacterial Activity, Phytochemical Analysis
To cite this article
Orowale Kehinde Nathaniel, Agina S. E., Evaluation of the Antibacterial Activity of Seed Coat Extracts of Roselle (Hibiscus Sabdariffa L.) on Selected Antibiotic Resistant Bacterial Species, Journal of Plant Sciences. Vol. 3, No. 5, 2015, pp. 259-263. doi: 10.11648/j.jps.20150305.13
Todar, K. (2007). Pathogenic E. Coli online textbook of Bacteriology. University of Wisconsin.
Center for Dieases Control and Prevention (2014) New England Journal of Medicine.
Quarenghi, M. V., Teres Chuk, M. L, Bangori M. D. and Abdala L. R. (2000) Antimicrobial activity of flowers from Anthemis cotula Fitoterupia 71:710-2.
Zhang, Q. H., Zhang, B. (2007). Research advance in chemical composition and Pharmapcolyiod of Chrysanthemum inorifolium: Food and drug Journal, 9: 60-2.
Zhao, L. Yang, L. Liu, Y. Lic, and Kang, W. (2009). Antimicrobial activity of seven species Chrysanthemum morifolium ramat cultivated in kaifeng. Modern Pharmacy Research, 2: 82-85.
Bako, I. O. G. Mabrouk, M. A and Abubaker, A. (2009). Antioxidant effect of ethanolic seed extract of Hibiscus sabdariffa linn (Malvaceae) to Alleviate the toxicity included by chronic administration of sodium Nitrate on some Hematological parameters in wistars Rats. Advanced Journal of Food Science and Technology 1(1): 39-42.
Chewonarin, T., Kinouchi, T., Kataoka, K., Arimachi, H., Kuwahara, T., Initkekumnven, U. and Ohnishi, Y. (1999). Effects of roselle (Hibiscus sabdariffa linn) a Thai medicinal plant, on the mutagencity of various known mutagenesis in Salmonella typhimurium and on formation of aberrant crypt foci induced by the colon carcinogens azoxymethane and 2-amind-methyl-6-phenylimidazo (4, 5-6) pyridine in F 344 rats. Food and Chemical Toxicology, 37, 591-601.
Cheesbrough, Monica. (2000). District laboratory practice in tropical Countries, part 2. Cambridge University press. 132-143.
Sofowora A (1993) phytochemical screening of medicinal plants and traditional medicine in Africa, 2nd edition, spectrum books ltd. Nigeria. pp 150-156.
Evans WC (2002) Trease and evan’s pharmacognosy 5th ed Haacourt Brace and Company pp336.
Rhoades, David F (1979) “Evolution of Plant Chemical Defense against Herbivores” in Rosenthal, Gerald A., and Janzen, Daniel H. herbivores: their interaction with secondary plant metabolites. New York Academic Press. P, 41.
Suffredini IB, Paciienca ML, Nepomucano DC, Younes RN, Veralla AD (2006) Anti microbial and cytotoxic activity of some brizillan plant extracts clusiacea Men. Inst. Oswaldocruz. riode janeiro 101(3): 287-287.
Tsuchiya H, Saro M, miyaziki T, Fujiwara S, Ohyama M, Tannaka M(1996) Comperative study on antibacterial activity of phytochemical flavanones methicillin-resistant staphylococcus aureus. Journal Ethanopharmacol 50: 27- 48.
Cowan, M.M (1999). Plant products as antimicrobial agents: Clinical Microbiology Reviews.12: 564-82.
Grotewold, E. (2006) The Science of Flavonoid. New York: Springer publisher, p1- 280.
Tapsell, L. C., I. Hemphill, L. Cobiac, C. S., Patch and D. R. Sulivan. (2006) “Health benefit of herbs and species”. The past, the present the future medical Journal Australia, 185:53-524.
Brownlee, H. E, McEven AR., Hedger J. and Scott I. M. (1990). Antifungal effects of cocoa tannin on the witches broom pathogen Crinipellis penieosa. Physiology Molecular Plant Pathology, 36:39-48.
Jang, D. S., Han, A. R; Min, H. Y., Windono, T. Jeohn, G. H: Lee, S. K, and Seo E. K (2004). “A new cytotoxic phenylbutenoid dimmer from the rhizomes of zingiber cassumunar. Planta medica. Volume 70(11); P. 1095-1097.
Nwaiwu, N. E, Mishelia, F. and Raufu, I. A. (2012) “Antimicrobial activities of crude extracts of Moringa oleifera, Hibiscus sabdariffa and Hibiscus esculentus seeds against some enterobacteria”. Journal of Applied Phytotechnology in Environmental sanitation, 1: 11-16.
Lessoy, T. Z., Micae’ E. B., Jean, T. G., Betty, M. F. and Sebastian, L. N. (2012) Two novel non-conventional seed oil extracts with antioxidant an antimicrobial activities. Tropical Journal of Pharmaceutical Research, 11(3); 469-475.
Khalid S. Abd-Ulgadir, Suliman, I. Suliman, Ismail A. Zakria and Nasr El-Deen A. Hassan (2015) Antimicrobial potential of methanolic extracts of Hibiscus sabdariffa and Ricinus communis. Advancement in Medicinal Plant Research vol, 3(1), pp18-22.
Browse journals by subject