Volume 3, Issue 2, April 2015, Page: 85-91
Molecular Cloning, Characterization and Expression Analysis of MhRAR1 Gene from Malus Hupehensis
Zhang Ji-Yu, Institute of Botany, Jiangsu Province and the Chinese Academy of Sciences, Nanjing, China
Guo Zhong-Ren, Institute of Botany, Jiangsu Province and the Chinese Academy of Sciences, Nanjing, China
Received: Mar. 9, 2015;       Accepted: Mar. 22, 2015;       Published: Mar. 26, 2015
DOI: 10.11648/j.jps.20150302.17      View  2707      Downloads  155
A novel RAR1 gene, designated MhRAR1, was cloned by the methods of RT-PCR and RACE from Malus hupehensis. The full length sequence of MhRAR1 is 1065 bp with an open reading frame of 678 bp, encoding a protein of 225 amino acids. As found in other plant RAR1 proteins, sequence alignment showed that MhRAR1 protein contains two CHORD domains and one plant-specific CCCH domain. In addition, the MhRAR1 contains conserved strings of invariant cysteine and histidine residues within the CHORD domains and CCCH domain. These results suggested that MhRAR1 protein from M. hupehensis might share the similar function with the Arabidopsis thaliana RAR1 and Hordeum vulgare RAR1, and is an important component of R gene–mediated disease resistance. Phylogenetic analysis revealed that MhRAR1 was closely related to Ricinus communis RAR1. The analysis by qRT-PCR revealed that the expression of MhRAR1 gene was higher in leaves than that in stems and roots. SA, MeJA and ACC treatment induced MhRAR1 expression in stems and roots, but not in leaves. Expression of MhRAR1 was weakly induced in M. hupehensis after infection with Botryosphaeria berengeriana. The cloning and characterization of the MhRAR1 gene will be useful for further studies of biological roles of MhRAR1 in plants.
Malus hupehensis, MhRAR1, cDNA Cloning, Expression Pattern
To cite this article
Zhang Ji-Yu, Guo Zhong-Ren, Molecular Cloning, Characterization and Expression Analysis of MhRAR1 Gene from Malus Hupehensis, Journal of Plant Sciences. Vol. 3, No. 2, 2015, pp. 85-91. doi: 10.11648/j.jps.20150302.17
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