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Extraction and Purification of Flaxseed Proteins and Studying their Antibacterial Activities

Received: 25 December 2013     Published: 28 February 2014
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Abstract

Flaxseed or linseed (Linum usitatissimum L.) is an annual herb belongs to the Linaceae family. It is cultivated worldwide and has been used for its oil seed and fiber since ancient times in Egypt, Rome and Greece. Nowadays it is considered as a medicinal plant in Asia, Europe and North America. Therapeutic effects of flaxseed fixed oil have been reported in several publications, but there are only few reports on biological activities of the flaxseed proteins. In the present study antimicrobial activities of the flaxseed proteins have been investigated. Hemogenized flaxseed flour was at first defatted and demucilaged, and its protein content was extracted in distilled water. After centrifugation, proteins of supernatant were isolated by adjusting pH. Gel filtration and ion-exchange chromatography on the protein extact gave fairly pure protein fractions, which antibacterial activities were examined against several microorganisms using the microbial micro-plate dilution method. The results showed that flaxseed proteins have an inhibitory activity on bacteria especially against Entrococcus foecalis, Salmonella typhimurium and Escherichia coli. Our experiment also revealed that mucilage composition as carbohydrates would not contribute in the inhibitory effect, but in fact, might cover and inhibit the antibacterial activity of flaxseed proteins. Moreover, it is possible that the carbohydrate components of flaxseed promote the microorganism growth.

Published in Journal of Plant Sciences (Volume 2, Issue 1)
DOI 10.11648/j.jps.20140201.21
Page(s) 70-76
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Linum Usitatissimum, Linseed, Flaxseed, Antimicrobial Activity, Protein Purification, Sephadex Gel Filtration, Ion Exchange, SDS-PAGE

References
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  • APA Style

    Mohammad Hassan Houshdar Tehrani, Rumeysa Batal, Mohammad Kamalinejad, Arash Mahbubi. (2014). Extraction and Purification of Flaxseed Proteins and Studying their Antibacterial Activities. Journal of Plant Sciences, 2(1), 70-76. https://doi.org/10.11648/j.jps.20140201.21

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    ACS Style

    Mohammad Hassan Houshdar Tehrani; Rumeysa Batal; Mohammad Kamalinejad; Arash Mahbubi. Extraction and Purification of Flaxseed Proteins and Studying their Antibacterial Activities. J. Plant Sci. 2014, 2(1), 70-76. doi: 10.11648/j.jps.20140201.21

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    AMA Style

    Mohammad Hassan Houshdar Tehrani, Rumeysa Batal, Mohammad Kamalinejad, Arash Mahbubi. Extraction and Purification of Flaxseed Proteins and Studying their Antibacterial Activities. J Plant Sci. 2014;2(1):70-76. doi: 10.11648/j.jps.20140201.21

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  • @article{10.11648/j.jps.20140201.21,
      author = {Mohammad Hassan Houshdar Tehrani and Rumeysa Batal and Mohammad Kamalinejad and Arash Mahbubi},
      title = {Extraction and Purification of Flaxseed Proteins and Studying their Antibacterial Activities},
      journal = {Journal of Plant Sciences},
      volume = {2},
      number = {1},
      pages = {70-76},
      doi = {10.11648/j.jps.20140201.21},
      url = {https://doi.org/10.11648/j.jps.20140201.21},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20140201.21},
      abstract = {Flaxseed or linseed (Linum usitatissimum L.) is an annual herb belongs to the Linaceae family. It is cultivated worldwide and has been used for its oil seed and fiber since ancient times in Egypt, Rome and Greece. Nowadays it is considered as a medicinal plant in Asia, Europe and North America. Therapeutic effects of flaxseed fixed oil have been reported in several publications, but there are only few reports on biological activities of the flaxseed proteins. In the present study antimicrobial activities of the flaxseed proteins have been investigated. Hemogenized flaxseed flour was at first defatted and demucilaged, and its protein content was extracted in distilled water. After centrifugation, proteins of supernatant were isolated by adjusting pH. Gel filtration and ion-exchange chromatography on the protein extact gave fairly pure protein fractions, which antibacterial activities were examined against several microorganisms using the microbial micro-plate dilution method. The results showed that flaxseed proteins have an inhibitory activity on bacteria especially against Entrococcus foecalis, Salmonella typhimurium and Escherichia coli. Our experiment also revealed that mucilage composition as carbohydrates would not contribute in the inhibitory effect, but in fact, might cover and inhibit the antibacterial activity of flaxseed proteins.  Moreover, it is possible that the carbohydrate components of flaxseed promote the microorganism growth.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - Extraction and Purification of Flaxseed Proteins and Studying their Antibacterial Activities
    AU  - Mohammad Hassan Houshdar Tehrani
    AU  - Rumeysa Batal
    AU  - Mohammad Kamalinejad
    AU  - Arash Mahbubi
    Y1  - 2014/02/28
    PY  - 2014
    N1  - https://doi.org/10.11648/j.jps.20140201.21
    DO  - 10.11648/j.jps.20140201.21
    T2  - Journal of Plant Sciences
    JF  - Journal of Plant Sciences
    JO  - Journal of Plant Sciences
    SP  - 70
    EP  - 76
    PB  - Science Publishing Group
    SN  - 2331-0731
    UR  - https://doi.org/10.11648/j.jps.20140201.21
    AB  - Flaxseed or linseed (Linum usitatissimum L.) is an annual herb belongs to the Linaceae family. It is cultivated worldwide and has been used for its oil seed and fiber since ancient times in Egypt, Rome and Greece. Nowadays it is considered as a medicinal plant in Asia, Europe and North America. Therapeutic effects of flaxseed fixed oil have been reported in several publications, but there are only few reports on biological activities of the flaxseed proteins. In the present study antimicrobial activities of the flaxseed proteins have been investigated. Hemogenized flaxseed flour was at first defatted and demucilaged, and its protein content was extracted in distilled water. After centrifugation, proteins of supernatant were isolated by adjusting pH. Gel filtration and ion-exchange chromatography on the protein extact gave fairly pure protein fractions, which antibacterial activities were examined against several microorganisms using the microbial micro-plate dilution method. The results showed that flaxseed proteins have an inhibitory activity on bacteria especially against Entrococcus foecalis, Salmonella typhimurium and Escherichia coli. Our experiment also revealed that mucilage composition as carbohydrates would not contribute in the inhibitory effect, but in fact, might cover and inhibit the antibacterial activity of flaxseed proteins.  Moreover, it is possible that the carbohydrate components of flaxseed promote the microorganism growth.
    VL  - 2
    IS  - 1
    ER  - 

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Author Information
  • Medicinal Chemistry Department, Shahid Beheshti University of Medical Sciences, Tehran, Iran

  • Medicinal Chemistry Department, Shahid Beheshti University of Medical Sciences, Tehran, Iran

  • Pharmacognosy Department, Shahid Beheshti University of Medical Sciences, Tehran, Iran

  • Pharmaceutics Department, Shahid Beheshti University of Medical Sciences, Tehran, Iran

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